pGLO

pGLO Observations , Data Recording & Analysis

Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB carpet white white - pGLO LB/amp 0 white white + pGLO LB/amp 150~ white white + pGLO LB/amp/ara 70~SO white green glowing(only 30~ colonies glow)

	In our test, the bacteria gained the traits of ampicillin resistance, and the pGLO gene, which makes them glow green when exposed to arabinose sugar.
	We spread 100uL of bacteria on the petri dish, and in that 100uL estimate that there are roughly 1500 bacterium in 100uL because there are 150 colonies on the the one with ampicillin ,and the gene. There are at least to times as many bacterium on the plate with only lori broth, so the must be atlast 1500 bacterium in the 100uL.
	The arabinose sugar allows the GFP gene to be repressed. When it is present, it attaches to the repressor for the GFP gene, and prevents it from attaching to the GFP operator, which allows the GFP gene to be expressed. The GFP gene can be used to tell if an organism has also taken a gene the will not be expressed. You can tell if it absorbed insulin gene, if it glows green. It can also let you follow the movement of substances in an animal, and track the progress of cancer through a brain. One other example of genetic engineering is our turkeys. The have been bred over the years to become as plump as possible.